Neuroendocrine control of planarian regeneration(RNAi scRNA-seq)

Effective tissue regeneration requires cells to perceive the surrounding context and make internal state adaptations that enable context-dependent fate changes in time and space, but the mechanisms underlying this complex process remain unclear. Here, transcriptom sequencing was used for study neuroendocrine control of planarian regneration Overall design: Examine single-cell gene expression profiles on 3dpa planarian tail fragments after EGFP RNAi(control), nsf RNAi and scg5 RNAi.