Sex differences in the synaptic genomic fabrics of the rat hypothalamic paraventricular node

We profiled the whole transcriptomes of male and female rat hypothalamic paraventricular nodes to determine whether there are sex differences in the topology of the genomic fabrics responsible for the glutamatergic, GABAergic, dopaminergic, cholinergic and serotonergic transmission. Our Genomic Fabric Paradigm (GFP) is proposed as a transformative research approach to enhance the understanding of the brain transcriptomic circuitries. The genomic fabric of a particular synapse is the structured transcriptome associated with the most interconnected and stably expressed gene network responsible for that type of neurotransmission. GFP refines the description of functional pathways by selecting the most prominent genes and determining their networking. Moreover, it quantifies the remodeling of functional pathways and their interplay in disease and recovery in response to a treatment. This experiment provides the absolute controls for a larger study on a rat model of autism spectrum disorder and its treatment with ACTH and PMX53. One tissue (hypothalamic paraventricular node) x two sexes (M, F) . Biological replicates: 4 PSNSM, 4 PSNSF.