Modification of specificity of non-coding RNA molecules to silence gene expression in eukaryotic cells

Eukaryotic cells-provided that the eukaryotic cells are not plant cells-encode non-coding RNA molecules that do not have RNA silencing activity or are processed into the non-coding RNA molecules A method of modifying a gene to be disclosed is disclosed. The method comprises introducing a DNA editing agent into a eukaryotic cell that confers the silencing specificity of the non-coding RNA molecule toward the target RNA of interest. Includes. Also disclosed are methods of encoding RNA silencing molecules for target RNAs in eukaryotic cells or modifying genes that are processed into the RNA silencing molecules. Also disclosed are methods of preventing and treating diseases， methods of inducing cell death， and methods of producing eukaryotic non-human organisms. A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell， with the proviso that said eukaryotic cell is not a plant cell， is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule to a target RNA in a eukaryotic cell is also disclosed. Methods of disease prevention and treatment， methods of inducing cell apoptosis and methods of generating a eukaryotic non-human organism are also disclosed. A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell， with the proviso that said eukaryotic cell is not a plant cell， is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule to a target RNA in a eukaryotic cell is also disclosed. Methods of disease prevention and treatment， methods of inducing cell apoptosis and methods of generating a eukaryotic non-human organism are also disclosed.