Gene expression profiling of WT and Aldh1a1 KO mouse lung tissues

To explore potential impact of Aldh1a1 deficiency in the retinoic acid signaling in the lung, gene expression levels were compared between wildtype and Aldh1a1 deficient lung tissues. The expression levels of retinoic acid responsive genes were very similar between wildtype and Aldh1a1 deficient lungs, suggesting that Aldh1a1 deficiency has minimal impact on retinoic acid signaling. Lung tissues were isolated from two mice per group (Aldh1a1+/+ and Aldh1a1-/-) and enzymatically digested. Extracted and purified total RNA samples were used for RNA-seq analysis.