five

Analysis of neonatal B lymphocytes

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39489
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B lymphocytes in the neonates are poorly define and some of which express the marker CD5. The experiment is based on the flow cytometry cell sorting of 2 populations of B lymphocytes defined according to their phenotype : 1/ CD5 positive sorted as CD20+CD19+CD3-CD5+CD10- ; 2/ CD5 negative B lymphocytes sorted as CD20+CD19+CD3-CD5-CD10-. Cells were isolated from fresh heparinised cord blood used within 24 hours after sample collect, without any additional treatment Comparison of CD5-positive and CD5-negative B lymphocytes isolated from cord blood obtained from 4 donors
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2017-06-07
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