RNA profiles of polysomes from MOLM-13 cells treated with FLT3 inhibitor and/or mTOR inhibitor in the presence of conditioned media of bone marrow stomal cells

RNA-seq analysis of MOLM13 cells to compare mRNAs enriched in heavy polysomes post FLT3 inhibition and/or mTOR inhibition in the presence or absence of conditioned media of bone marrow stromal cells HS-5 (hBMSC-CM) MOLM-13 cells were treated with FLT3 inhibitor AC220 (quizartinib) and/or mTOR inhibitor everolimus in the presence or absence of hBMSC-CM for 12 hours. Post drug treatments, cells were harvested for polysome profiling. Using input RNAs and mRNAs enriched in heavy polysomes, we performed transcriptome analysis.