Human Cord Blood Erythroid Cell Immune Transcriptome
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Extract protocol We isolated cord blood mononuclear cells using density gradient centrifugation with a density of 1.077 g/mL at 266 RCF for 30 min. We then extracted human erythroid cells by CD235-positive magnetic separation. Library construction protocol The scRNAseq was performed using the BD Rhapsody™ System mRNA Targeted and Sample Tag Library Preparation Protocol Data processing Sample Tag-demultiplexed immune transcriptome data was obtained via BD Rhapsody pipeline ver. 1.10 from R1 and R2 FASTQ files Reference Panel BD Rhapsody™ Immune ResponseTargeted Panel (Human) (https://scomix.bd.com/hc/article_attachments/13766899446029).
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Zenodo创建时间：
2025-02-03
