General results of the primary screening and validation.

N indicates the number of positive hits in respective families. Gaussia Luciferase (GLuc) activity and gene expression were expressed as fold induction compared to the average signal of control wells. P value was based on luminescence assay for GLuc activity (n = 4). Toxicity was evaluated by Alamar Blue assay and DNA content. The hit selection resulting from the primary screen was validated in a repeat of the screen including a dose-response (confirmed positives).