Exploring the role of TAGLN in the pathogenesis of pathological scarring

Pathological scarring is a fibroproliferative disorder characterised by abnormal fibroblast function and excessive deposition of extracellular matrix. In this study, based on the results of preliminary single-cell sequencing, we identified an enriched fibroblast subpopulation in keloids, which highly expresses TAGLN. In this study, we investigated the roles of TAGLN in the pathogenesis of pathological scars, respectively. And functional assays revealed that downregulation of TAGLN inhibited the motility activity and secretory functions of pathological scar fibroblasts, including invasion, migration, contraction and collagen secretion. We identified downstream targets of TAGLN by RNA-seq results analysis and further validation in. This study provides new perspectives for understanding pathological scarring and possible targets for clinical treatment. Overall design: To elucidate the potential mechanisms of TAGLN in pathological scarring, we performed RNA-seq sequencing of HPSF after treatment with si-TAGLN and si-NC. We first analysed the gene expression of each group in the RNA sequencing data and identified differentially expressed genes based on a P value less than or equal to 0.01. And we combined GO enrichment analysis and KEGG analysis to screen potential downstream targets.