Effect of blocking ABA-signaling in different tissues on transcriptomic changes induced by ABA-treatment

ABA-signaling in disitinct root tissues is important for xylem phenotypes observed both under mock and ABA-treatment. We performed RNA-sequencing on Arabidopsis plants in which ABA-signalling is specifically blocked in the endodermis (J0571), the xylem (J1721) or the procambium (Q0990) to investigate the underlying transcriptomic changes. Overall design: Transcriptomic changes in 1cm roots of 5 day old Arabidopsis plants upon treatment with 1µM ABA/ mock for 8h. Plants used in this experiments are in the F1 generation from the cross of the UAS::abi1-1 line with different enhancer trap lines: UAS::abi1-1xC24 (wt), UAS::abi1-1xJ1721, UAS::abi1-1xJ0571, UAS:abi1-1xQ0990