Mode matrix R after the first iteration step (left) converting reaction R2 from numerical to binary form and after the last iteration step (right) for a regulated EFM analysis.

After the final step R contains only binary values. Note that in the last step regEFMtool calculates fewer modes than the ordinary EFMtool (see Table 3). The italic font type highlights reactions disobeying the rule R7r = NOT(R9). After the first step M5 (highlighted in bold font) disobeys the iteration phase rule and is removed from the matrix. After the final step M8, M9, and M10 do not obey the iteration phase rule. Additionally, M1 and M7 (also highlighted in bold font) disobey the post-processing rule. M5 is also removed, as it is a futile 2-cycle mode created by splitting the reversible reaction R7r into two irreversible reaction.