Wound healing assay data

In wound-healing system a scratch was generated in subconfluent layers of cells and the repopulation of the denuded areas was measured. High miR-92b expression accelerated wound closure by 63.97% at 24h and by 66.96% at 48h after transfection in comparison with negative control group in PCI-37A cells. Additionally, an apparent reduced repopulation area was observed at 24h and 48h after transfection in miR-92b knockdown group. Similar results were also obtained from the experiments performed with PCI-37B cells.Wound healing assay in PCI-37A cells revealed that the healing percentage of SP1 siRNA group was decreased by 15.47% at 24h and by 26.51% at 48h in comparison with negative control group, respectively.

在伤口愈合实验体系中，于亚汇合态的细胞单层制作划痕，并对裸露区域的细胞再填充情况进行定量检测。在PCI-37A细胞中，与阴性对照组相比，高表达微小RNA-92b（miR-92b）可使划痕愈合率在转染后24小时提升63.97%，48小时时提升66.96%。此外，miR-92b敲低组在转染后24小时及48小时均可观察到细胞再填充区域显著减少。采用PCI-37B细胞开展的实验也得到了一致结果。对PCI-37A细胞开展的伤口愈合实验结果显示，与阴性对照组相比，SP1小干扰RNA（SP1 siRNA）组的划痕愈合率在转染后24小时降低15.47%，48小时时降低26.51%。