Comparative analysis data of the qPCR amplification curves of BoR300 repeats and the single copy gene (ace), for the absolute estimation of BoR300 copies.

a,bIntercept and slope of the linear regression lines (Figure 6).cThe efficiency of the reaction was estimated by the equation: E = 10−1/slope-1.dΔCt value is obtained by comparing the y-axis intercepts derived from the two lines-of-best-fit of the target sequences BoR300 and ace.eAccording to the mean efficiency of the reactions (94.05%) the amplification factor F was 1.881.