RNA-sequencing analysis of mouse epithelial NMuMG cells with Cas9 or UGCG knock out

We report the gene expression in mouse NMuMG cells with or without UGCG depletion using RNA sequencing. After data analysis, the 11218 transcripts were identified. Among these transcripts, the TGF-ß signaling target genes and EMT marker genes were significantly upregulated in UGCG depleted NMuMG cells. Then we used these genes and mouse TGF-ß or EMT gene signatures to do the gene set enrichment analysis and showed the negative correlation of UGCG and TGF-ß signaling or EMT in NMuMG cells. Overall design: RNA profiles of mouse NMuMG cells with Cas9 (as the control group) and UGCG knock out. Each group contains three biological replicates.