Single-cell RNA sequencing of human airway epithelia infected with measles virus (MeV)

The goal of this study was to compare cell composition, gene expression, and infectivity of different cell types in human airway epithelia following exposure to measles virus. Samples included control epithelia exposed to a mock infection and measles-virus-exposed epithelia that were sorted according to detection of green fluorescent protein (GFP) prior to library preparation and sequencing. Overall design: Primary basal cells were collected from lung donors, infected with Measles Virus, prepared for single-cell sequencing via 10X Genomics Chromium controller, and sequenced on Illumina NovaSeq6000.