Sirolimus induced phosphaturia

Male Wistar rats received sirolimus or vehicle for 7 days (1.5mg/kg) and were placed in individual metabolic cages for eight days allowing a 24 hour adaption period to the metabolic cage environment. At the end of the experiments rats were anesthetized by inhalation of Isoflurane/air and blood samples and kidneys were collected.雄性Wistar大鼠连续7天接受剂量为1.5mg/kg的西罗莫司（sirolimus）或赋形剂（vehicle）处理。给药完成后，将大鼠单笼置于代谢笼中饲养8天，其中包含24小时的代谢笼环境适应期。实验结束后，通过吸入异氟烷（Isoflurane）/空气对大鼠实施麻醉，采集血液样本与肾脏组织。