Comparative RNA-sequencing of Brachypodium distachyon wild-type and bdmute mature leaf zones

Comparative RNA-sequencing of the mature leaf zones in Brachypodium distachyon wild type and bdmute mutants that do not form stomatal subsidiary cells was performed. The aim was to identify genes relevant for subsidiary cell function in B. distachyon. Overall design: 25 mature zones (25-30mm from base of 2nd leaves) were analyzed per replicate and genotype. 3 replicates per genotype were sequenced, WT1, WT2, WT5 and sid1, sid2, sid5. WT corresponds to the reference genotype and sid (=bdmute) corresponds to the mutant genotype.