Histone demethylase KDM4A-mediated promotion of PAF1/NFATC2 expression is required for oncogenesis in MLL-AF9 acute myeloid leukemia [RNA-seq]

In this study we identified the histone demethylase KDM4A as an essential and selective regulator of AML oncogenic potential. RNAseq was used to follow the changes in gene expression following KDM4A knockdown in MLL-AF9 human AML THP-1 cells Overall design: Human MLL-AF9 AML THP-1 cells were spinoculated with two separate and distinct lentiviruses, each resulting in the expression of a shRNA targeting the KDM4A transcript. A third lentivirus was included as a negative control, which causes the expression of a non-targeting control (NTC) shRNA. Puromycin selection was used to select cells which had been infected 48 hours following spinoculation. Total RNA was harvested 72 hours following puromycin selection. There are biological replicates for each condition.