Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction

To advance our understanding of the dynamics of the gene regulation during each step of in vitro HSPC, we surveyed global gene expression, chromatin accessibility (ATAC-seq), chromatin immunoprecipitation sequencing (ChIP-seq) for active H3K4me3 and repressive H3K27me3 histone mark, from hPSCs and purified intermediates across HSPC differentiation. Methods: ATAC-seq, bulk RNA-seq, scRNA-seq, ChIP-seq, scATAC-seq, Cut & Tag Bulk RNA-seq, ATAC-seq, H3K4me3 and H3K27me3 ChIP-seq were performed with hPSCs and hPSC derived VMEs, EPCs and HPCs . Besides, we performed scATAC-seq and scRNA-seq to investigate the transcriptomic signature of HEC generated in in vitro system.