N-linked glycosylation of recombinant Porcine Delta Coronavirus spike protein ectodomain from drosophila S2 cells

Recombinant PDCoV spike glycoprotein was produced from Drosophile S2 cells for structural characterization by cryo electron microscopy and mass spectrometry. MS was used to assess the N-linked glycosylation profile of the purified material. We used a multiple protease digestion in combination with RP-LC-MS/MS, EThcD fragmentation on Orbitrap Fusion to characterize intact glycopeptides.