RNA-seq analysis after genetic overexpression or knockdown of TP73 in TL-Om1 cells
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RNA-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze gene expression changes after genetic overexpression or knockdown of TP73. Overall design: For overexpression study, lentiviral infection was carried out on TL-Om1 cells to overexpress TAp73 or ?Np73 under doxycycline (dox)-inducible system. For knockdown study, lentiviral infection was carried out on TL-Om1 cells to stably express dCas9 and dox-inducible sgRNA targeting TP73 locus. Total RNA was harvested 3 days after dox induction and RNA-seq analysis was performed.
创建时间：
2023-11-22
