Figure 4

PDF file: Uncropped blots used in final figures for PSD95, synaptophysin and actin loading control in 5xFAD and TgA-5xFAD male mice 3-5 months old. Figure 4 Sheet 1 (b): Silver staining (20 x magnification) quantification of number of silver particles (analyzed from thresholded image) across the whole hippocampus 5xFAD and TgA-5xFAD mice. Three-four sagittal sections from at least four 5xFAD or TgA-5xFAD mice were used for all staining and immunostaining experiments. Figure 4 Sheet 2 and 3 (d and e): Raw densitometric quantifications of synaptic markers PSD95 (sheet 1) and synaptophysin (sheet 2) in cortical lysates from 3-5-month old 5xFAD and TgA-5xFAD male mice densitometric quantification normalized to actin loading control. Figure 4 Sheet 4 (g): Raw data for percent area quantification of cleaved caspase-3 immunofluorescence percent area across the hippocampus. Figure 4 Sheet 5 and Sheet 6: h Percentage time spent in each quadrant searching for removed escape platform in the Morris water maze in the probe trial (after 4 days of training to find an escape platform). Mice were given 60 s to explore the pool. Two days following the Probe trial, animals were put on the “Reversal” phase of the task, in which the target platform was placed in a different quadrant. Animals were trained for four days then memory was probed again and is denoted as the i Reversal probe. T= Target, O= quadrant Opposite to target quadrant, L= quadrant to the Left of the target quadrant, R= quadrant to the Right of the target quadrant.