Micron Zero-valent Iron Chitosan Hydrogel Balls Boosts Nitrate Removal in Constructed Wetlands for Secondary Effluent Treatment

Reducing nitrate in the secondary effluent from municipal wastewater treatment plants (WWTPs) can prevent eutrophication, which can be achieved by constructed wetlands. Zero-valent iron (ZVI) has been used as electron donors for nitrate removal in constructed wetlands to deal with the low carbon and nitrogen ratio (C/N) problem, but the effects are often limited by passivation. In this study, micron zero-valent iron chitosan hydrogel balls (MZVI@CS) were prepared as part of the substrate. The total nitrogen (TN) removal efficiency maintained at 85%-96% in 70 days. The chelating ability of chitosan could reduce the formation of iron oxides on the surface of iron particles and microbial cells, thus eliminating the passivation. Denitrification microorganisms were enriched and the expressions of denitrification genes were increased. The study provides new understandings of further improving the nitrate removal efficiency of constructed wetlands under low C/N and efficient use of iron materials.