Impact of purified Cry1Ac, Cry2Ab and E-64 provided in artificial diet on survival and development of Coleomegilla maculata.

Larvae of C. maculata were fed an artificial diet containing 100 µg Cry1Ac, 400 µg Cry2Ab, 100 µg Cry1Ac and 400 µg Cry2Ab, or 150 µg E-64 (positive control) per g fresh weight of artificial diet. Pure diet served as a negative control (N = 30). The experiment lasted until adult emergence.Statistical comparisons were made separately for each of the insecticidal proteins with the control. Asterisks denote significant differences:**P*Chi-square test with Bonferroni correction (adjusted α = 0.017).†Mann-Whitney U-test with Bonferroni correction (adjusted α = 0.017).‡Dunnett test.