PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent - Supplementary dataset

Aim: To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA
polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents.
Methods: A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyICPBNPs
were stable and monodisperse, maintained the native absorbance properties of both polyIC and
PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies.
Results: PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of
HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free
polyIC in CD4 and CD8 T cells. Conclusion: PBNPs function as efficient carriers of nucleic acids to directly
reverse HIV latency and enhance immune activation.

研究目的：探究普鲁士蓝纳米颗粒（PBNPs）表面涂覆dsRNA的合成类似物多聚肌苷酸-多聚胞苷酸（polyIC）的能力，作为HIV潜伏期逆转剂。研究方法：采用层-by-层技术合成polyIC涂覆的PBNPs（polyIC-PBNPs）。polyIC-PBNPs稳定性良好，呈现单分散性，保留了polyIC和PBNPs的原生吸收特性，且以高纳米颗粒收集产量和polyIC附着效率获得。研究结果：在HIV潜伏期细胞模型中，polyIC-PBNPs在激活潜伏HIV方面比游离的polyIC更为有效。此外，polyIC-PBNPs在促进CD4和CD8 T细胞的免疫激活方面也比游离的polyIC更为有效。研究结论：PBNPs作为高效的核酸载体，可直接逆转HIV潜伏期并增强免疫激活。