Homatula variegata isolate:YT-2025 Genome sequencing

we present the first telomere-to-telomere (T2T) nuclear genome assembly of P. variegatus, generated using a hybrid approach combining PacBio HiFi long reads, Oxford Nanopore ultra-long reads, and Hi-C chromatin conformation capture. A total of 24 chromosomes were assembled, with a genome size of 641.26 Mb and an N50 value of 24.40 Mb. This assembly fills a critical gap in genomic resources for Nemacheilinae and represents the first T2T genome for any species within the suborder of Cobitoidei. The genome provides a valuable reference for advancing genetic improvement, resource management, and aquaculture development of P. variegatus as a native ornamental and economic species.