Sevoflurane Exposure Alters Long Non-coding RNA and Messenger RNA Profiles in the Neonatal Mouse Hippocampus

The focus of current study is to investigate the effect of developing sevoflurane exposure on lncRNA profiles and molecular signaling involvement in neurotoxicity. An Expression Microarray assay (V3.0) and data analysis services were provided by Arraystar Inc. (Rockville, MD) to assess and compare the global abundance of 27,427 lncRNAs and 18,855 mRNAs in the hippocampi of mice exposed to either sevoflurane or normal air controls. Postnatal day 7 (PD7) mice were exposed to either 3% sevoflurane or normal air for 6 hours, n=4. RNA was isolated from the hippocampus, converted to cDNA, synthesized from RNA via reverse transcription, and hybridized to Arraystar’s custom probes.