The mechanism of Sinomenine (SIN) ameliorates the metabolic-associated steatohepatitis (MASH)

The alkaloid, sinomenine (SIN),7,8-didehydro-4-hydroxy-3,7-dimethoxy-17-methylmorphinan-6-one (C19H23NO4), was extracted from the rhizome of the Chinese medicinal plant, Sinomenium acutum (Thunb.) Rehd. et Wils. andS. acutum (Thunb.) Rehd. et Wils. Var. cinereum Rehd. et Wils.SIN has antiinflammatory, analgesic,immunosuppressive,antihypertensive, and antiarrhythmic effects. It has been used totreat rheumatoid diseases in humans.Our laboratory has shown that SIN can attenuate the inflammation in dextran sulfate-induced colitis, but it is unclear in metabolic-associated steatohepatitis (MASH). Overall design: Once the AML12 cells reached confluency , they were stimulated with FFAs for 24h, and then stimulated with FFAs and 200µM sinomenine for 48h