Analysis of the effect of cofactor knockdown on dexamethasone-induced gene expression in B-cell acute lymphoblastic leukemia

After identifying cofactors that affected the sensitivity of dex-induced cell death in B-ALL, we knocked down three cofactors, NCAO2, EHMT1, and EHMT2, using shRNAs in NALM6 cells After measuring the effect of cofactor knockdown on dex-sensitivity of these cells, we looked at differences in gene expression after exposure to dexamethasone. NALM6 cells were treated with vehicle (0.1% ethanol) or dexamethasone (1µM) for four hours, then harvested. The samples are unmodified NALM6 cells, NALM with a scrambled shRNA sequence, and the three cofactor knockdowns.