RT-PCR confirms novel transcripts and shows their presence in testis and FHSD patient cells.

1“+” symbols indicate that an RT-PCR product was obtained and verified by Sanger sequencing. “−” symbols indicate samples that were either negative, or gave only bands derived from unrelated loci (our primers recognize repetitive elements, so clean amplification is sometimes difficult). “(+)” symbols indicate that an RT-PCR product of the expected size was obtained, but could not be cloned for sequencing. No products were obtained from corresponding negative control samples prepared without reverse transcriptase.