Catherine Galbraith, James Galbraith (2010) CIL:7053, Mus musculus, permanent cell line cell. CIL. Dataset

NIH 3T3 cell expressing EGFP-Lifeact (a small genetically expressed probe that binds to actin and is derived from the first 17 aa of Abp140) imaged with structured illumination. Structured illumination produces images that have a lateral resolution of approximately 100 nm, or twice the diffraction limited 200 nm resolution of conventional fluorescent microscopy techniques.

There are three related images in this folder. This file is the raw data. The 5 positions of the illumination grid pattern are visible in the 10 slices of the z-series. The structure of this file is that all 150 images appear as a horizontally incrementing sequence. The second file is a z-series of six of the reconstructed structured illumination images. The third file contains the maximum projection image of the second file. The second and third files each have 4 channels of data to compare image resolution: channel 1 - structured illumination, channel 2 - wide field, channel 3 - deconvolved wide field, and channel 4 - filtered deconvolved wide field.

Images were collected with a Carl Zeiss SIM microscope using a 63 X 1.4 Plan Apo objective, an Andor Ixon camera with 150 ms exposures,and Zeiss filter set 2.

美国国立卫生研究院（NIH）3T3细胞表达EGFP-Lifeact（一种小型的基因表达探针，可与肌动蛋白结合，并源自Abp140的前17个氨基酸残基）的图像，采用结构照明进行成像。结构照明技术生成的图像具有约100纳米的横向分辨率，是传统荧光显微镜技术衍射极限的200纳米分辨率的两倍。该文件夹中包含三幅相关图像。本文件为原始数据。在z序列的10个切片中可见照明网格的5个位置。该文件的结构为所有150幅图像均呈现为水平递增的序列。第二个文件为六个重构结构照明图像的z序列。第三个文件包含第二个文件的极大值投影图像。第二个和第三个文件各包含4通道数据，以比较图像分辨率：通道1 - 结构照明，通道2 - 宽场，通道3 - 解卷积宽场，通道4 - 滤波解卷积宽场。图像使用卡尔·蔡司SIM显微镜，配备63倍1.4计划阿波物镜、安道尔IXON相机，曝光时间为150毫秒，以及蔡司滤光片组2进行采集。