Data From: The 1014F knockdown resistance mutation is not a strong correlate of phenotypic resistance to pyrethroids in Florida populations of <i>Culex quinquefasciatus</i>

<i>Culex quinquefasciatus</i> is an important target for vector control because of its ability to transmit pathogens that cause disease. Most populations are resistant to pyrethroids and often to organophosphates, the two most common classes of active ingredients used by public health agencies. A knockdown resistance (<i>kdr)</i> mutation, resulting in a change from a leucine to phenylalanine in the voltage gated sodium channel, is one mechanism contributing to the pyrethroid resistant phenotype. Enzymatic resistance has also been shown to play a very important role. Recent studies have shown strong resistance in populations even when <i>kdr</i> is relatively low which indicates factors other than <i>kdr</i> may be larger contributors to resistance. In this study, we examined on a statewide scale (over 70 populations), the strength of the correlation between resistance in the CDC bottle bioassay and the<i> kdr</i> genotypes and allele frequencies. Spearman correlation analysis showed only moderate (-0.51) and weak (-0.29) correlation between the <i>kdr</i> genotype and permethrin and deltamethrin respectively. The frequency of the <i>kdr</i> allele was an even weaker correlate. These results indicate, in contrast to <i>Aedes aegypti</i>, assessing <i>kdr</i> in populations of <i>Culex quinquefasciatus</i> is not a good surrogate for phenotypic resistance testing.致倦库蚊（Culex quinquefasciatus）是媒介防制的重要靶标物种，因其可传播致病性病原体。该蚊绝大多数种群对拟除虫菊酯类（pyrethroids）与有机磷类（organophosphates）杀虫剂产生抗性，而这两类正是公共卫生机构应用最为广泛的两类有效成分杀虫剂。 导致电压门控钠离子通道上亮氨酸突变为苯丙氨酸的击倒抗性（knockdown resistance，kdr）突变，是介导拟除虫菊酯抗性表型的机制之一。酶介导抗性同样被证实对抗性的产生发挥了极为关键的作用。 近期研究发现，即便种群的kdr突变频率相对较低，仍可表现出较强的抗性，这提示除kdr之外的其他因素可能对抗性的贡献更大。 本研究在全州范围内（覆盖70余个种群），探究了CDC瓶式生物测定法所测得的抗性水平与kdr基因型及等位基因频率之间的相关性强度。斯皮尔曼相关性分析结果表明，kdr基因型与氯菊酯、溴氰菊酯的抗性水平分别呈现中等程度（-0.51）与较弱程度（-0.29）的相关性；而kdr等位基因频率与抗性的相关性则更为微弱。 上述结果表明，与埃及伊蚊（Aedes aegypti）不同，通过检测致倦库蚊种群的kdr突变，无法很好地替代表型抗性检测。