Table 4 in Detection and characterization of diverse coccidian protozoa shed by California sea lions

Table 4 Characterization of zoites grown in MA104 cell cultures inoculated with sporulated, excysted oocysts of Coccidia A and B from California sea lion (CSL-) fecal samples 10089, 10092, 10100 and 10266. Antigenic reactivity of zoites to Sarcocystis neurona antisera from a horse and a sea lion (CSL 9878) by indirect fluorescent antibody test (IFAT). PCR amplification of zoite DNA confirmed that zoites were similar to S. neurona (Sn).
CSL10089CSL10092CSL10100CSL10266MA104 (control)Fecal oocyst coccidian sequence type aAA & BA & BANTZoites observed in MA104 cell culture+++––IFAT results with antigen slides of CSL zoites in MA104 cell cultureS. neurona antiseraHorse 6814411:3201:1601:320NTNTCSL98781:10,2401:12801:640NTNTPCR results with approximate amplicon size (coccidian species/genotype b)Genetic locus/primersITS-1 (pan-coccidian) c1000 bp (Sn)1000 bp (Sn)/ 400 bp1000 bp (Sn)/400 bp (Coccidia A)Negative(Coccidia A)400 bp (Coccidia A + B)ITS1500 (S. neurona / falcatula)500 bp (Sn)500 bp (Sn)500 bp (Sn)NegativeNegativeSn7150 bp (Sn/CA17)150 bp (Sn/CA17)150 bp (Sn/CA17)NTNTSn9150 bp (Sn/GT18)150 bp (Sn/GT18)150 bp (Sn/GT18)NTNTSn-ITS600 bp (Sn)600 bp (Sn)600 bp (Sn)NTNTSnSAG 1-5-61000 bp (Sn)1000 bp (Sn)1000 bp (Sn)NTNTSnSAG31000 bp (Sn)1000 bp (Sn)1000 bp (Sn)NTNTSnSAG41000 bp (Sn)1000 bp (Sn)1000 bp (Sn)NTNT
NT = not tested.
a Coccidian species determined based on analysis of DNA from frozen pelleted oocsyts from feces and not culture inoculum, using ITS-1 locus PCR and sequencing.
b Based on genetic similarity across unambiguous sequence.
c 400 bp amplicon was only observed the first 1 ‾ 2 wks post-inoculation. The 1000 bp PCR products were amplified continuously in CSL10089, CSL10092, and CSL10100 cultures.