Table 3 in A revised taxonomy and phylogeny of opalinids (Stramenopiles: Opalinata) inferred from the analysis of complete nuclear ribosomal DNA genes

Table 3. List of sequences used in the present study. Sequences lacking>100 bases at the 5 ʹ or 3 ʹ ends are shown in parentheses. New sequences are marked in bold.
Sequence/speciesAccession sequenceNumberLengthGC%SourceSSU rDNASTRAMENOPILESOPALINATAOpalinida(Protoopalina intestinalis (Stein* 1856) Metcalf* 1923)AY576545140933.6Kostka et al. (2004)(Protoopalina axonucleata Metcalf * 1923a)AB175929126332.5Nishi et al. (2005)Protoopalina limnocharisMN625264182932.2This studyProtoopalina pingiMN625266183032.4This studyZelleriella orientalisMN625263214326.4This studyZelleriella sp.MN625265214326.4This studyOpalina undulataMF434114216726.8Li et al. (2018)Opalina triangulataMK872804216626.8Wang et al. (2019)Opalina obtrigonoideaMK970648216726.8Zhao et al. (2020)Opalina japonicaMN625267217026.0This studyOpalina longaMN625268217127.7This studyProteromonadidaKarotomorpha sp.DQ431242185844.1Kostka et al. (2007)(Karotomorpha sp.)DQ431243142446.2Kostka et al. (2007)Proteromonas lacertae Grassé * 1879U37108174337.4Leipe et al. (1996)BLASTOCYSTIDABlastocystis cycluri Singh et al. * 1996AY266474184835.6Yoshikawa et al. (2004)Blastocystis lapemi Teow et al. * 1991 Blastocystis pythoni Singh et al. * 1996AY266471 AY2664721840 179436.7 39.1Yoshikawa et al. (2004) Yoshikawa et al. (2004)Blastocystis hominis (Swaine & Britain* 1849) Brumpt* 1912Blastocystis ratti Chen et al. * 1997 PLACIDIDAAB023499 AY5901141740 173043.6 40.3Arisue et al. (2002) Noel et al. (2005)Placidia cafeteriopsis Moriya et al. * 2002JF834161177549.4Chan YF* Chen CW* Tsai AY* Chiang KP* unpublishedSuigetsumonas clinomigrationis Okamura & Kondo * 2015Wobblia lunata Moriya et al. * 2000 BICOSOECIDAAB976561 AB0326061769 172652.0 49.1Okamura and Kondo (2015) Moriya et al. (2000)Bicosoeca petiolata (Stein* 1878) Prinsheim* 1946AY520444180948.6Cavalier-Smith and Chao (2006)Cafeteria roenbergensis Fenchel & Patterson * 1988AF174364169547.1Atkins et al. (2000)ALVEOLATAAPICOMPLEXABabesia gibsoni (Patton* 1910)KC461261166545.5Mandal et al. (2015)Eimeria necatrix Johnson * 1930KT184349176346.8Ogedengbe et al. (2016)DINOFLAGELLATABlastodinium contortum Chatton * 1908DQ317537179944.8Skovgaard et al. (2007)Prorocentrum arenarium Faust * 1994Y16234178846.4Grzebyk et al. (1998)CILIOPHORALItonotus paracygnus Song* 1994EU242509163542.0Gao et al. (2008)Paramecium buestchlii van As et al. * 1998KM091234170344.2Krenek et al. (2015)AMORPHEACHOANOZOASalpingoeca infusionum Kent * 1880AF100941197849.6Collins (1998)ITS region (OPALINATA)Protoopalina limnocharisMN630239This studyITS12312.25.8S15435.7ITS22294.4Protoopalina pingiMN630241This studyITS12372.15.8S15436.4ITS22184.6Zelleriella orientalisMN630236This studyITS12912.85.8S16726.4ITS22852.5Zelleriella sp.MN630237This studyITS12902.85.8S16626.5ITS22792.5Opalina undulataMG816209Li et al. (2018)ITS13251.55.8S16724.0ITS22902.4Opalina triangulataMK872803Wang et al. (2019)ITS12921.75.8S16724.6ITS22841.8Opalina obtrigonoideaMK973090Zhao et al. (2020)ITS13231.65.8S16724.6ITS22871.7Opalina japonicaMN630238This studyITS13571.75.8S16724.6ITS23582.5Opalina longaMN630240This studyITS12831.85.8S16724.6ITS22612.3LSU rDNA (OPALINATA)Protoopalina limnocharisMN638759365226.7This studyProtoopalina pingiMN638761364726.9This studyZelleriella orientalisMN638760406423.0This studyZelleriella sp.MN638763410222.8This studyOpalina undulataMN638758410822.8This studyOpalina triangulataMN638762404323.9This studyOpalina obtrigonoideaMW504710412222.5This studyOpalina japonicaMN638764412022.4This studyOpalina longaMN638765409623.6This study
aNishi et al. (2005: 701–702) indicated that ‘the four sequences from Protoopalina sp. were all identical* although they were isolated at different geographical locations.Futhermore* the genus Protoopalina is presently restricted to the host species Rana nigromaculata in Japan.They commonly retain four relatively large nuclei* show similar morphological characters (Table 1)* and are classified as P.japonica (Hara 1934) ’. However* the work by Hara (1934) is on the cell morphology and microtubular structures of Protoopalina axonucleata * and he did not mention any other species in his work.According to the statements by Nishi et al. (2005) * their isolates corresponded to the same species studied by Hara (1934) and should be identified as P.axonucleata. The name Protoopalina japonica is a nomen nudum because this species has never been described.