File S1 - Abnormal Interactions between Perifollicular Mast Cells and CD8+ T-Cells May Contribute to the Pathogenesis of Alopecia Areata

Background: S1–S2. Material and methods: S3. Result: S4–S7. Discussion: S8–S9. References. Figure S1. Positive controls for triple-immunostainings. Figure S2. MC density is significantly increased in lesional skin compared to non-lesional skin of AA patients and scalp skin from healthy subjects. The number of proliferating c-Kit+ MC is tendentially increased in AA lesional skin compared to control skin. Figure S3. The maximal increase of MC density is found in AA patients in subacute stage of the disease. Figure S4. TGFβ1 immunoreactivity is decreased in the HF ORS of AA patients. Figure S5. MHCI/CD8/Tryptase triple staining showing MHCI+ MCs in close contact with CD8+ T-cells. Table S1. Primary antibody. Table S2. Expression of pro-inflammatory and pro-inhibitory molecules and cytokines which are considered to be involved in the cross-talk between MCs and CD8+ T-cells in PFD of lesional AA skin compared to non-lesional AA and healthy skin.
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