Jennifer Bestman, Holly Cline (2011) CIL:37082, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #2 (hour 2) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour intervals over 8 hours, and shows the development and morphological plasticity of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为发育中的兔属无色斑蛙视束中枢神经元图像，编号为第2张（2小时阶段）。该细胞在8小时内每隔2小时进行一次成像，展示了树突树和轴突随时间推移的发展与形态可塑性。细胞在图像获取前约24小时通过活体电穿孔法转染编码增强型绿色荧光蛋白（eGFP）的质粒DNA。在获取图像时，完整的活体蝌蚪被麻醉，放置在载玻片下，并通过定制构建的双光子显微镜直接透过其透明皮肤进行成像。