Single-cell gene expression of control mandibular bone marrow cells and mandibular bone marrow cells under apical periodontitis

Single-cell gene expression of mandibular bone marrow cells and mandibular bone marrow cells under the stimulation of apical periodontitis were determined by scRNAseq. Twenty C57/B6 male mice with apical periodontitis or control mice were used to acquire single-cell suspensions. Mandibles were dissected under a stereo microscope. Soft tissues, molars, incisors and bone behind the condyle were removed. Then, mandibular alveolar bone tissue was digested to acquire single cell suspension. After red blood cells were lysed, cellular suspensions were loaded into Chromium microfluidic chips and barcoded with a 10x Chromium Controller (10x Genomics).