Effects of overexpression of IFI6 in bone marrow mesenchymal stem cells on gene expression profiles of acute lymphoblastic leukemia cells. Effects of overexpression of IFI6 in bone marrow mesenchymal stem cells on gene expression profiles of acute lymphoblastic leukemia cells

We explored the effects of high expression of interferon-inducible protein 6 in bone marrow mesenchymal stem cells on the gene expression profiles of acute lymphoblastic leukemia cells. Overall design: The human acute lymphoblastic leukemia (ALL) cell lines RS4;11 were obtained from the Laboratory of Hematopoietic Stem Cell Transplantation Center of Guizhou Province and were cultured in an RPMI-1640 medium. Bone marrow mesenchymal stem cells (BM-MSCs) were isolated from bone marrow aspirates of ALL patients and were cultured in the complete medium of adult BM-MSCs (Saiye, Shanghai, China). RS4;11 cell lines (n=3) co-cultured with MSCs transfected with empty vector (EV) or MSCs transfected with interferon-inducible protein 6 (IFI6) overexpression clone lentiviral particle (LV-IFI6) for 72 h were collected, respectively. Then RNA sequencing analyses were performed on RS4;11 cell lines in the two groups.