Changes in tumor infiltrating leukocytes following TA99 CAR T cell treatment

Tumor infiltrating leukocytes were analyzed by scRNAseq 9 days after treatment with TA99 (anti-TRP-1) CAR T cells with or without Super2 and IL-33 co-expression. To identify changes in tumor infiltrating CAR T cells and host leukocytes, C57BL/6 mice inoculated with 2 x 10^5 B16F10 cells were treated with 7x10^6 CAR T cells after 6 days, and then tumors were harvested on day 15, digested and purified by percoll gradient prior to single cell RNA-seq and Antibody-derived tags.