Whole Transcriptome Sequencing (mRNA-seq) of cultured pulmonary primary endothelial cells treated with or without H2O2

ROS once released from myeloid cells is quickly converted into H2O2 in lungs. We performed RNAseq of cultured pulmonary primary endothelial cells treated with or without H2O2. Pathway enrichment analysis revealed that some of the signaling pathways that are altered by H2O2 are related to AKT signaling. AKT signaling has a protective role in a murine model of ALI by preventing capillary leakage. Moreover, H2O2 stimulates AKT activation in endothelial cells to strengthen vessel barrier integrity mRNA profiles of cultured pulmonary primary endothelial cells treated with or without H2O2, in triplicate, using Illumina.