Ionizing radiation induced hair and skin damage

Skin samples from back region and footpads were analyzed in 8-10 week old mice. For back skin, the hair was plucked in order to induce active growing phase(anagen). After 9 days, the mice were irradiated for 5Gy IR. Krt17 knock out, p53 knock out, and wild type mice were compared. Samples were taken at time 0 (control), 6h, and 24h post-irradiation. For footpad skin, the mice were irradiated for 40 Gy IR. Krt17 knock out and wild type mice were compared. Samples were taken at time 0 (control), 3 d, 6 d, 9 d post-irradiation. Overall design: Expression profiling by high-throughput sequencing