Serum proteomic signatures in non-human primates following treatment with a radiation countermeasure and exposure to a partial- or total-body supralethal radiation dose

As countries face emerging conflicts and evaluate military strategies, the potential for nuclear-related accidents continues to rise, putting large populations of civilians at risk. As a result, the development of pharmaceuticals that can be administered prior to radiation exposure to protect from radiation-induced injury is of utmost importance. However, there are currently no prophylactic drugs that can be used to protect against radiation injury. One drug under advanced development, gamma-tocotrienol (GT3), has proved to be promising in terms of its antioxidant activity as well as its accelerated hematopoietic recovery and reduction of DNA damage in treated animals exposed to various doses of ionizing radiation. In this study, nonhuman primates (NHPs) were leveraged to investigate the protective effects of GT3 on proteomic profiles in conjunction with a supralethal dose (12 Gy) of either total-body irradiation (TBI) or partial-body irradiation (PBI), performed with 5% bone marrow spa..., , # Serum proteomic signatures in non-human primates following treatment with a radiation countermeasure and exposure to a partial- or total-body supralethal radiation dose

Dataset DOI: [10.5061/dryad.9ghx3ffx0](10.5061/dryad.9ghx3ffx0)

## Description of the data and file structure

A total of 32 NHPs (rhesus macaques) were used for this study; 16 animals were exposed to partial-body radiation (LINAC X-ray radiation, 1.3 Gy/min) and the remaining 16 animals were exposed to total-body radiation (cobalt-60 γ-radiation, 0.6 Gy/min). In each group, eight animals were administered either vehicle or 37.5 mg/kg of GT3 subcutaneously (*sc*) 24 h prior to irradiation. Blood samples were collected from NHPs undergoing partial- or total-body irradiation (PBI or TBI) at the following time points: 3 d prior to irradiation and 4, 8, 12 h, 1, 2, and 6 d post-irradiation. Serum was isolated and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a data-independent acquisition...,