Figure 4. Difopein, an antagonist of 14-3-3/ligand interactions, does not affect Nav1.5-conducted sodium current.

Figure 4. Difopein, an antagonist of 14-3-3/ligand interactions, does not affect Nav1.5-conducted sodium current. (a) Current density-voltage (IV) relationships from Nav1.5-expressing tsA201 cells transiently expressing CD8 with empty vector, difopein, or mutant of difopein. (b) Representative whole-cell INa traces recorded from the listed conditions. (c) Peak current densities of each group. (d) Activation (A) and steady-state inactivation (SSI) curves. (e) Fast inactivation constant (τfast) plotted as a function of test potential. (f)  Reversal potential (Vrev). (g) Half-voltage of SSI (V1/2 SSI). (h) Half-voltage of A (V1/2 A). Values pertaining to the biophysical properties are shown in Table 5. Data are presented as mean ± SEM. n, number of cells taken for analysis. Kruskal-Wallis with post hoc Dunn's multiple comparisons has been performed in (c, f, g, and h) and the multiplicity adjusted p-values are indicated in each panel.