A Characterization of the Chromatin Landscape and the Glucocorticoid Receptor Cistrome in Mouse Cerebral Cortical Embryonic Neural Stem and Progenitor Cells

The goals of this study are to use assays for transposase accessible chromatin and dual-crosslinking immunoprecipitation, each followed by genome-wide sequencing (ATAC-seq and ChIP-seq, respectively) to measure chromatin accessibility and genomic GR binding patterns in vehicle-treated and dexamethasone-treated primary embryonic neural and progenitor stem cell cultures. (1) ATAC-seq on female primary embryonic (E14.5) neural and progenitor stem cell neurosphere cultures. 2 conditions: ethanol-treated and dexamethasone-treated (10-7M) for a total of 4 hours.There are three individual biological replicates for each condition. (2) ChIP-seq on female primary embryonic (E14.5) neural and progenitor stem cell neurosphere cultures. 2 conditions: ethanol-treated and dexamethsone-treated (10-7M) for a total of 4 hours. 1-2 seperate embryos were pulled evenly between treatment conditions to obtain sufficient cell counts. There are three technical replicates for each condition.