Effects of PHGDH CRISPR knockout on gene expression in glioblastoma-associated endothelial cells
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP365774
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资源简介：
PHGDH was knocked out in human glioblastoma (GBM)-associated endothelial cells (ECs) using CRISPR-Cas9/gRNA. RNA was isolated and analyzed by RNAseq. The effects of PHGDH knockout on gene expression were determined. Overall design: ECs were isolated from GBM tumors and transduced with the lenvirus expressing PHGDH sgRNA or control sgRNA, followed by cell selection by incubation with medium containing 2 µg/ml puromycin. RNA was isolated and analyzed by RNAseq.
创建时间：
2025-05-17
