An evolutionarily conserved transcription factor controlling leaf vein growth in flowering plants (VDof1 ChIP-seq)

The veins of leaves are a highly evolved vascular network that allows plants to grow large and complex. The patterning process leading to their formation involves the integration of several internal and external signals, such as plant hormone auxin. Here, we show that an evolutionarily conserved transcription factor controlling leaf vein growth in flowering plants VDOF1 is dependent on autophagy for its activity in Arabidopsis thaliana leaf, suggesting that this pathway might be required for proper vascular system development in leaf. Taken together, our data forms that during leaf vein patterning there is presents a network in which a module that links VDOF1-ATG8-ANT1-SCR-SHR factors integrates Space-time dimension to provide more vein density, which establish a precondition for C4 photosynthesis transition. Overall design: ChIP-seq was used to investigate the effect of VDof1 overexpression on the Arabidopsis thaliana genome. The results showed that VDof1 binds to a number of target genes, which may be involved in its regulatory effects.