Whole genome bisulfite sequencing of mouse DC subsets

Diet plays an important role in lifestyle disorders and our study from Methionine Choline deficient diet induced non-alcoholic fatty liver disease led to specific decrease in pDC fraction from murine spleens. Delineating the role for individual components identified correlation of L-methionine supplementation with representation of pDC subtype. Considering implication of L-methionine in maintaining methylation status, we investigated the DNA methylation profiles among DC subtypes. Whole genome bisulfite sequencing analysis identified that pDCs display distinctly methylated regions in comparison to cDC subsets whereas comparable methylation in cDC1 and cDC2 served as a control. Further analysis of the pathways associated with DMRs, we reported that inhibition of MAPK pathway guides DC development towards pDC subtype. We demonstrate that supplementation with S-adenosylmethionine but not Homocysteine reversed the defect in pDC development induced by methionine deficiency. Overall, our study identifies important role for methionine in pDC biology. Examination of DNA methylation profile of pDC, cDC1 and cDC2 subsets from murine spleens