Identification of genetic loci participating in K. pneumoniae AJ218 biofilm formation.

aIdentification of transposon insertion sites in K. pneumoniae AJ218 were derived from homology searches of the K. pneumoniae NTUH-K2044 genome sequence (GenBank Ref: AP006725).bExpressed as a percentage of the biofilm produced by K. pneumoniae AJ218Rif, set to 100%. Determined using the static microtiter plate assay following incubation in M63B1-GCAA (supplemented with 1% glycerol and 0.3% casamino acids) minimal media for 24 h at 37°C. Values represent the mean ± standard deviation, of four replicate sample wells for each strain performed in two independent experiments.

K. pneumoniae AJ218中转座子插入位点的鉴定，源自对K. pneumoniae NTUH-K2044基因组序列（GenBank参考号：AP006725）的同源搜索。b以K. pneumoniae AJ218Rif产生的生物膜百分比为表达，设定为100%。通过在37°C下M63B1-GCAA最小培养基（补充1%甘油和0.3%可溶性蛋白氨基酸）中培养24小时，并使用静态微量滴定板法进行测定。所列数值代表四个重复样品孔的平均值±标准偏差，每个菌株均在两个独立的实验中进行测定。