Estrogen Receptor beta cistrome in mouse endometriotic tissue.

Estrogen Receptor beta (ERβ) has an essential role in endometriosis progression. However, the role of genomic ERβ in the pathogenesis of endometriosis is not elucidated, yet. To get ERβ cistrome, we employed endometrium specific ERβ overexpression (ERBOE) mouse model by crossing mouse having pCAG promoter-loxPSTOPloxP-ERβ cassette with PRCre knockin mice that Cre recombinase cDNA was inserted into exon 1 of PR gene. Using ERBOE mouse model, we determine ERβ-cistrome in endometriotic lesions and eutopic endometrium of mice with endometriosis. ERβ cistrome analysis in ectopic lesions: We isolated uteri from ERBOE female mice (eight weeks old) and then made endometrium fragments by using Miltex Medical Biopsy Punch Dermal 2 mm OR Grade. The endometriosis was surgically induced by implanting one uterus fragment from ERBOE female mouse to wild-type intact C57BL/6J mouse (n=9, eight weeks old). At estrus cycle in 4th weeks of endometriosis induction, we harvested ectopic lesions and then pooled them. Uaing pooled ectopic lesions; we conducted the ERβ-ChIP Seq. ERβ cistrome analysis in eutopic endometrium: We isolated uteri from wild-type C57BL/6J female (eight weeks old) and then made endometrium fragments by using Miltex Medical Biopsy Punch Dermal 2 mm OR Grade. The endometriosis was surgically induced by implanting one uterus fragment isolated from wild-type C57BL/6J into ERBOE female mouse (eight weeks old) (n=9, eight weeks old). At estrus cycle in 4th weeks of endometriosis induction, we isolated eutopic endometrium and then pooled them. Using pooled eutopic endometria, we conducted the ERβ-ChIP Seq.