Enforcement of stem-cell dormancy by nucleophosmin mutation is a critical determinant of unrestricted self-renewal during myeloid leukemogenesis

The most frequent mutation in AML patients with normal karyotype affects the Nucleophosmin gene (NPMc+). Different mouse models have shown that NPMc+ mutation can drive leukemia development. In this study, the impact of NPMc+ expression on the transcriptional program of mouse hematopietic stem cells (HSC) in vivo has been investigated. RNA was purified from controls and NPMc+ expressing FACS sorted LT-HSC and subjected to microarray analysis. Three biological replicates have been performed.